Extinction coefficients of bacteriochlorophylls d and e in organic solvents for quantitative spectrophotometric determination of pigments of phototrophic green sulphur bacteria
Zhiltsova A. A. 1, Krasnova E. D. 2, Voronov D. A.3, Sokolovskaya Y. G. 1, Patsaeva S. V. 1
1Department of Physics, Lomonosov Moscow State University, Moscow, Russia
2Department of Biology, Moscow State University, Moscow, Russia
3Kharkevich Institute for Information Transmission Problems, Russian Academy of Sciences, Moscow, Russia
Email: aa.zhiljtcova@physics.msu.ru, e_d_krasnova@wsbs-msu.ru, da_voronov@mail.ru, sokolovskaja.julija@physics.msu.ru, spatsaeva@mail.ru

PDF
The extinction coefficients of bacteriochlorophylls d and e in several organic solvents were determined and compared with the coefficients of related compounds - chlorophylls a and b. In some solvents (ethanol, isopropanol, acetone-ethanol (7 : 2) and acetone-methanol (7 : 2) - for bacteriochlorophyll d, in isopropanol and acetone-ethanol (7 : 2) - for bacteriochlorophyll e), extinction coefficients were determined for the first time. Spectrophotometric formulas for quantitative measurement of bacteriochlorophylls d and e in different solvents are given. The method of dynamic light scattering revealed the absence of noticeable aggregation of bacteriochlorophyll molecules in acetone and ethanol to a concentration of 0.14 g/l. The obtained data expand the possibilities of the spectrophotometric method for quantifying the pigments of phototrophic bacteria. Keywords: bacteriochlorophyll, absorption spectra, extinction coefficients, organic solvents, anoxygenic phototrophic bacteria.
  1. T.S. Gostev, F.I. Kouzminov, M.Yu. Gorbunov, E.N. Voronova, V.V. Fadeev. EARSeL eProceedings, 11 (21), 98 (2012)
  2. M.Y. Gorbunov, P.G. Falkowski. Limnology and Oceanography, 66 (1), 1 (2021). DOI: 10.1002/lno.11581
  3. F.I. Kuz'minov, E.A. Shirshin, M.Yu. Gorbunov, V.V. Fadeev. Fundam. Prikl. Gidrofiz., 8 (1), 41 (2015) (in Russian)
  4. K.R. Block, J.M. O'Brien, W.J. Edwards, C.L. Marnocha. Microbiology Open, 10 (4), e1228 (2021). DOI: 10.1002/mbo3.1228
  5. E.A. Kudryavtseva, T.V. Bukanova, S.V. Aleksandrov. Sovrem. Probl. DZZ Kosmosa, 19 (4), 59 (2022) (in Russian). DOI: 10.21046/2070-7401-2022-19-4-59-74
  6. A. Razjivin, J. Gotze, E. Lukashev, V. Kozlovsky, A. Ashikhmin, Z. Makhneva, A. Moskalenko, H. Lokstein, V. Paschenko. J. Phys. Chem. B, 125 (14), 3538 (2021). DOI: 10.1021/acs.jpcb.1c00719
  7. L. Limantara, S. Sakamoto, Y. Koyama, H. Nagae. Photochem. Photobiol., 65 (2), 330 (1997). DOI: 10.1111/j.1751-1097.1997.tb08566.x
  8. J.S. Connolly, E.B. Samuel, A.F. Janzen. Photochem. Photobiol., 36 (5), 565 (1982). DOI: 10.1111/j.1751-1097.1982.tb04417.x
  9. J.P. Thornber, R.J. Cogdell, R.E.B. Seftor, G.D. Webster. Biochim. Biophys. Acta (BBA) - Bioenergetics, 593 (1), 60 (1980). DOI: 10.1016/0005-2728(80)90008-0
  10. J. Linnanto, J. Korppi-Tommola. Phys. Chem. Chem. Phys, 2 (21), 4962 (2000). DOI: 10.1039/b004998k
  11. Chlorophylls and Bacteriochlorophylls: Biochemistry, Biophysics, Functions and Applications, ed. by B. Grimm, R.J. Porra, W. Rudiger, H. Scheer (Springer, Dordrecht, 2006), vol. 25, ch. 6, p. 79-94
  12. M. Taniguchi, J.S. Lindsey. Photochem. Photobiol., 97 (1), (2020). DOI: 10.1111/php.13319
  13. J. Goc, A. Dudkowiak, Z. Gryczynski, I. Gryczynski, B. Zelent, D. Fr ackowiak. J. Fluoresc., 11 (1), 53 (2001). DOI: 10.1023/a:1016699616249
  14. T.P. Causgrove, P. Cheng, D.C. Brune, R.E. Blankenship. J. Phys. Chem., 97 (21), 5519 (1993). DOI: 10.1021/j100123a011
  15. S.C.M. Otte, J.C. van der Heiden, N. Pfennig, J. Amesz. Photosynth. Res., 28 (2), 77 (1991). DOI: 10.1007/bf00033717
  16. A.G. Yakovlev, A.S. Taisova, Z.G. Fetisova. Vestn. Mosk. Univ. Ser. 16, 78 (2), 64 (2023) (in Russian)
  17. Advances in Photosynthesis and Respiration, ed. by B.R. Green, W.W. Parson (Kluwer Academic Publishers, The Netherlands, 2003), vol. 13, ch. 2, p. 29-81. DOI: 10.1007/978-94-017-2087-8_2
  18. Chlorophylls and Bacteriochlorophylls: Biochemistry, Biophysics, Functions and Applications, ed. by B. Grimm, R.J. Porra, W. Rudiger, H. Scheer (Springer, Dordrecht, 2006), vol. 25, ch. 1, p. 1-26
  19. J. Overmann, M.M. Tilzer. Aquatic Sciences, 51 (4), 261 (1989). DOI: 10.1007/bf00877171
  20. P.S. Emeliantsev, A.A. Zhiltsova, E.D. Krasnova, D.A. Voronov, V.V. Rymar, S.V. Patsaeva. Moscow University Physics Bulletin, 75 (2), 137 (2020). DOI: 10.3103/S0027134920020046
  21. A.A. Zhiltsova, E.D. Krasnova, A. Prosenkov, A.I. Pelaez Andres, D.A. Voronov, S.V. Patsaeva. Proc. SPIE, 12086, 1208603 (2021). DOI: 10.1117/12.2613667
  22. A.A. Zhiltsova, O.A. Filippova, E.D. Krasnova, D.A. Voronov, S.V. Patsaeva. Atmospheric and Oceanic Optics, 35 (5), 562 (2022). DOI: 10.1134/S1024856022050232
  23. C.Mazi\`ere, M. Bodo, M.A. Perdrau, C. Cravo-Laureau, R. Duran, C. Dupuy, C. Hubas, Science of The Total Environment, 802, 149787 (2022). DOI: 10.1016/j.scitotenv.2021.1497
  24. L. de Moura Sousa, F.S. Moreira, V.L. Cardoso, F.R.X. Batista. J. Water Process Engineer., 52, 103567 (2023). DOI: 10.1016/j.jwpe.2023.103567
  25. T. Miyatake, H. Tamiaki. J. Photochem. Photobiol. C: Photochem. Rev., 6 (2-3), 89 (2005). DOI: 10.1016/j.jphotochemrev.2005
  26. R.Y. Stanier, J.H.C. Smith. Biochim. Biophys. Acta, 41 (3), 478 (1960). DOI: 10.1016/0006-3002(60)90045-7
  27. C.M. Borrego, J.B. Arellano, C.A. Abella, T. Gillbro, L.J. Garcia-Gil. Photosynth. Res., 60 (2-3), 257 (1999). DOI: 10.1023/A:1006230820007
  28. A. Picazo, C. Rochera, E. Vicente, M.R. Miracle, A. Camacho. Limnetica, 32 (1), 139 (2013). DOI: 10.23818/limn.32.13
  29. B. Tian, Z. Sun, S. Shen, H. Wang, J. Jiao, L. Wang, Y. Hu, Y. Hua. Lett. Appl. Microbiol., 49 (6), 699 (2009). DOI: 10.1111/j.1472-765x.2009.02727.x
  30. M. Ruivo, P. Cartaxana, M. Cardoso, A. Tenreiro, R. Tenreiro, B. Jesus. Limnology and Oceanography: Methods, 12 (6), 338 (2014). DOI: 10.4319/lom.2014.12.338
  31. O.N. Lunina, A.S. Savvichev, V.V. Babenko, D.I. Boldyreva, B.B. Kuznetsov, T.V. Kolganova, E.D. Krasnova, N.M. Kokryatskaya, E.F. Veslopolova, D.A. Voronov, N.A. Demidenko, M.A. Letarova, A.V. Letarov, V.M. Gorlenko. Microbiology, 88 (1), 100 (2019). DOI: 10.1134/S0026261719010041
  32. R.J. Ritchie, S. Sma-Air. J. Appl. Phycol., 34 (3), 1577 (2022). DOI: 10.1007/s10811-022-02740-z
  33. N.A. Marnautov, L.Kh. Komissarova, A.B. Elfimov. Int. J. Prof. Sci., 10, 10 (2020)
  34. N.D. Bowles, H.W. Paerl, J. Tucker. Can. J. Fish. Aquat. Sci., 42 (6), 1127 (1985). DOI: 10.1139/f85-139
  35. E.D. Krasnova, M.V. Mardashova. Priroda, 1, 16 (2020) (in Russian). DOI: 10.7868/S0032874X20010020
  36. E.D. Krasnova. Water Resour., 48 (3), 427 (2021). DOI: 10.1134/S009780782103009X
  37. V.A. Zhezherya, T.P. Zhezherya, P.M. Linnik. Hydrobiol. J., 58 (2), 79 (2022). DOI: 10.1615/HYDROBJ.V58.I2.70
  38. A.S. Savvichev, V.V. Babenko, O.N. Lunina, M.A. Letarova, D.I. Boldyreva, E.F. Veslopolova, N.A. Demidenko, N.M. Kokryatskaya, E.D. Krasnova, V.A. Gaisin, E.S. Kostryukova, V.M. Gorlenko, A.V. Letarov. Environmental Microbiology, 20 (10), 3784 (2018). DOI: 10.1111/1462-2920.14384
  39. D. Grouzdev, V. Gaisin, O. Lunina, M. Krutkina, E. Krasnova, D. Voronov, R. Baslerov, P. Sigalevich, A. Savvichev, V. Gorlenko. FEMS Microbiology Ecology, 98 (10), (2022). DOI: 10.1093/femsec/fiac103
  40. A.A. Zhiltsova, A.V. Kharcheva, E.D. Krasnova, O.N. Lunina, D.A. Voronov, A.S. Savvichev, O.M. Gorshkova, S.V. Patsaeva. Atmospheric and Oceanic Optics, 31 (4), 390 (2018). DOI: 10.1134/S1024856018040188
  41. Z.B. Namsaraev. Microbiology, 78 (6), 794 (2009). DOI: 10.1134/S0026261709060174
  42. J.G. Ormerod, T. Nesbakken, S.I. Beale. J. Bacteriol., 172 (3), 1352 (1990). DOI: 10.1128/jb.172.3.1352-1360.1990
  43. Phytoplankton Pigments in Oceanography: Guidelines to Modern Methods, ed. by S.W. Jeffrey, R.F.C. Mantoura, S.W.E. Wright (UNESCO Publishing, Paris, 1997), p. 595-596
  44. J.F.G.M. Wintermans, A. Demots. Biochim. Biophys. Acta, 109 (2), 448 (1965). DOI: 10.1016/0926-6585(65)90170-6
  45. Chlorophylls and Bacteriochlorophylls: Biochemistry, Biophysics, Functions and Applications, ed. by B. Grimm, R.J. Porra, W. Rudiger, H. Scheer (Springer, Dordrecht, 2006), vol. 25, ch. 7, p. 95-107. DOI: 10.1007/1-4020-4516-6_7
  46. Anoxygenic Photosynthetic Bacteria, ed. by R.E. Blankenship, M.T. Madigan, C.E. Bauer (Kluwer Academic Publishers, NY., 2004), vol. 2, ch. 20, p. 399-435
  47. A. Jensen, O. Aasmundrud, K.E. Eimhjellen. Biochim. Biophys. Acta, 88 (3), 466 (1964). DOI: 10.1016/0926-6577(64)90089-0
  48. A. Gloe, N. Pfennig, H. Brockmann, W. Trowitzsch. Arch. Microbiol., 102 (1), 103 (1975). DOI: 10.1007/BF00428353
  49. J.A. Maresca, A.G.M. Chew, M.R. Ponsati, N.-U. Frigaard, J.G. Ormerod, D.A. Bryant. J. Bacteriol., 186 (9), 2558 (2004). DOI: 10.1128/JB.186.9.2558-2566.2004

Подсчитывается количество просмотров абстрактов ("html" на диаграммах) и полных версий статей ("pdf"). Просмотры с одинаковых IP-адресов засчитываются, если происходят с интервалом не менее 2-х часов.

Дата начала обработки статистических данных - 27 января 2016 г.

Publisher:

Ioffe Institute

Institute Officers:

Director: Sergei V. Ivanov

Contact us:

26 Polytekhnicheskaya, Saint Petersburg 194021, Russian Federation
Fax: +7 (812) 297 1017
Phone: +7 (812) 297 2245
E-mail: post@mail.ioffe.ru