Extinction coefficients of bacteriochlorophylls d and e in organic solvents for quantitative spectrophotometric determination of pigments of phototrophic green sulphur bacteria
Zhiltsova A. A.
1, Krasnova E. D.
2, Voronov D. A.
3, Sokolovskaya Y. G.
1, Patsaeva S. V.
11Department of Physics, Lomonosov Moscow State University, Moscow, Russia
2Department of Biology, Moscow State University, Moscow, Russia
3Kharkevich Institute for Information Transmission Problems, Russian Academy of Sciences, Moscow, Russia
Email: aa.zhiljtcova@physics.msu.ru, e_d_krasnova@wsbs-msu.ru, da_voronov@mail.ru, sokolovskaja.julija@physics.msu.ru, spatsaeva@mail.ru
The extinction coefficients of bacteriochlorophylls d and e in several organic solvents were determined and compared with the coefficients of related compounds - chlorophylls a and b. In some solvents (ethanol, isopropanol, acetone-ethanol (7 : 2) and acetone-methanol (7 : 2) - for bacteriochlorophyll d, in isopropanol and acetone-ethanol (7 : 2) - for bacteriochlorophyll e), extinction coefficients were determined for the first time. Spectrophotometric formulas for quantitative measurement of bacteriochlorophylls d and e in different solvents are given. The method of dynamic light scattering revealed the absence of noticeable aggregation of bacteriochlorophyll molecules in acetone and ethanol to a concentration of 0.14 g/l. The obtained data expand the possibilities of the spectrophotometric method for quantifying the pigments of phototrophic bacteria. Keywords: bacteriochlorophyll, absorption spectra, extinction coefficients, organic solvents, anoxygenic phototrophic bacteria.
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