Gelatin Substrate for DNA Extraction
Germash N. N.1, Zaitseva M. V.1, Petrov D. G.1, Antifeev I. E.1, Makarova E. D.1
1Institute for Analytical Instrumentation of the Russian Academy of Sciences, Saint Petersburg, Russia
Email: yilatan_nata@mail.ru
The paper presents a method for creating a gel lifter using gelatin, glycerin, citric acid, and water. The efficiency of the developed gelatin gel lifters for collecting and subsequently extracting nucleic acids from various surfaces is studied. A comparison is made with traditional DNA collection methods, including cotton swabs and manual DNA extraction from E.coli samples. An assessment is made of the effect of the composition of the gel lifter substrates on their adsorption capacity. Keywords: gel lifters, gelatin, DNA extraction, collection and sample preparation of nucleic acids.
- D.G. Petrov, Razrabotka eksperimental'noi ustanovki dlya sozdaniya metodik avtomatizirovannogo vydeleniya nukleinovykh kislot na tverdoi faze, Candidate's Dissertation in Engineering (Inst. Anal. Priborostr. Ross. Akad. Nauk, SPb., 2022) (in Russian)
- D.G. Petrov, E.D. Makarova, I.E. Antifeev, M.V. Zaitseva, Nauchn. Priborostr., 34 (1), 81 (2024) (in Russian)
- C.M. Hymus, F.O. Baxter, H. Ta, T. Tran, C. de Sousa, N.S. Mountford, J.W. Tay, Leg. Med., 67, 102330 (2024). DOI: 10.1016/j.legalmed.2023.102330
- N. Suderman, M.I.N. Isa, N.M. Sarbon, Food Biosci., 24, 111 (2018). DOI: 10.1016/j.fbio.2018.06.006
- D. Hardman, T.G. Thuruthel, F. Iida, NPG Asia Mater., 14, 11 (2022). DOI: 10.1038/s41427-022-00357-9